The BRAIN Foundation
Synchrony Symposium 2023 Highlights
Synchrony 2023 was an in-person event, held this year on Oct 20-22 at the Asilomar Conference Grounds, Pacific Grove, California.
Synchrony covered many of the most cutting-edge topics in basic science and clinical research, with the speaker line up featuring leading academics, clinical practitioners, pharmaceutical industry leaders, venture partners and emerging biotech companies.
Some of the highlights included:
TALK BY A NOBEL LAUREATE – Mario Capecchi PhD, shared his amazing life’s journey – from a homeless street urchin at the age of 4 in wartime Italy to winning the Nobel Prize Winner in Medicine/Physiology in 2007.
KEYNOTE BY David Fajgenbaum, MD, MBA, MSc, co-founder of Every Cure and author of the best-seller ‘Chasing My Cure’. In 2010 David was diagnosed with Castleman Disease, which he was told was incurable. He nearly died five times, yet he didn’t give up – using his own samples, records and research he eventually discovered a new use for an existing drug. Now in remission for 8 years, he is on a mission to scale his approach and find cures for diseases that currently have none.
CLINICAL PEARLS SESSION and CME credits for physicians
CLINICAL & RESEARCH ROUNDTABLES – topics ranging from repurposed drugs to Transcranial Magnetic Stimulation, from biomarkers to organiods
ACTION PLAN – to get to standard of care for seizure management & neuropsychiatric exacerbations in autism
INDUSTRY TRACK – biotech and venture partners presented updates on therapeutics under development for FDAapproval
RESEARCHERS COURSE designed to inform, inspire and offer a springboard for young and upcoming researchers
MENTOR-MENTEE SESSIONS to foster cross-field interaction with leading experts, clinicians and researchers
Recordings of Synchrony 2023 presentations now available to watch here 🎥
▶️ Update on clinical trials of photobiomodulation light therapy for autism
▶️ Update on transcranial magnetic stimulation TMS for autism
▶️ Talk on exploring heterogeneity to advance precision medicine in autism
▶️ Keynote by David Fajgenbaum, MD, MBA, MSc, the author of the best-seller ‘Chasing My Cure’
And many others!
NEW: Earn CME credits by watching Synchrony videos!
We are delighted to now share The Pathways link for the video library (enduring content). Learners who have viewed the videos can use this link to launch into a separate portal (the accredited activity) to reflect on what they learned to earn credits. With this solution, they can use this link multiple times (to reflect on all the videos and various concepts that inspired their learning!).
This experience offers pathways to continuing education opportunities Dentists, Nurses, Nurse Practitioners, Pharmacists, Physician Associates, Physicians, Licensed Clinical Social Workers and other members of the healthcare team.
And many more 🎥
Synchrony 2023 Roundtables
- Roundtable 1: NEUROPSYCHIATRIC EXACERBATIONS IN ADOLESCENTS/ADULTS AND SEIZURES IN ASD - GETTING TO STANDARD OF CARE
- Discussion Summary
CHAIRS: John Gaitanis MD, Director of Child Neurology, Brown Medical School, Pediatric Neurologist, Epileptologist, Hasbro Children’s Hospital and Richard Frye MD, PhD
Behavioral Child Neurologist, Neurodevelopmental Precision Medicine
PANEL: Jennifer Frankovich MD MS, Clinical Professor, Dept of Pediatrics, Stanford Children’s Health, Lucile Packard Children’s Hospital, Stanford University; Harumi Jyonouchi MD, Allergist- Immunologist, St. Peter’s University Hospital; Arthur Krigsman MD, Pediatric Gastroenterologist, Private Practice, New York
COMING SOON
PANEL: Kim Hollingsworth Taylor, CEO, Clearly Present Foundation, Peter Enticott, PhD, Associate Dean, Research, Faculty of Health, Deakin University, Manpreet Kaur Singh, MD, MS, Associate Professor of Psychiatry and Behavioral Sciences, Stanford University, Nolan Williams, MD, Associate Professor of Psychiatry and Behavioral Sciences, Director, Stanford Interventional Psychiatry Clinical Research, Director, Stanford Brain Stimulation Laboratory, Co-Founder, Magnus Medical Inc., Ernest Pedapati, MD, MS, FAAP, Assistant Professor, Department of Psychiatry and Behavioral Neuroscience, University of Cincinnati, Isabella (Crys) Borsellino, Patient Experience, Student, UCSD, Developmental Psychology, Melissa Kirkovski, PhD, Research Fellow – iHES, College of Sport, Health, & Engineering, Victoria University
CHAIR: Alysson Muotri, PhD, Director, Stem Cell Program, UCSD, Professor, Departments of Pediatrics and Cellular & Molecular Medicine, UC, San Diego
PANEL: Giorgia Quadrato PhD, Assistant Professor of Stem Cell, Biology, Keck School of Medicine, University of Southern California; Alex Shcheglovitov, PhD, Assistant Professor, Neurobiology, University of Utah; Kristen Brennand PhD, Elizabeth Mears and House Jameson Professor of Psychiatry and Professor of Genetics, Yale University School of Medicine
TITLE: Single neural rosette-derived organoids as a tool for modeling human brain development and disease
ABSTRACT: Human brain development is unique, and we still have much to learn about the cellular and molecular mechanisms that underlie the specification and function of various cell types in the human brain. Moreover, it remains largely unknown how different cells and mechanisms are affected in neurodevelopmental disorders. We recently developed a new method for generating human telencephalic organoids from stem cell-derived single neural rosettes (SNR) and investigated the properties of cells in these organoids in health and disease (Wang, Chiola, et al., Nat Communication 2022). During my presentation, I will elaborate on our methodologies for generating SNR-derived organoids and discuss how we use these organoids for modeling neurodevelopmental disorders caused by specific genetic abnormalities.
KRISTIN BRENNAND, PHD
TITLE: Studying the genomics of brain disorders using stem cells
ABSTRACT: Each person’s distinct genetics and environment predispose them to some phenotypes and confers resilience to others. How do all the individual variants across the genetic landscape combine to yield larger phenotypic impacts in aggregate? How does genetic variation govern the penetrance of deleterious mutations, variable expressivity, and pleiotropy? What is the role of the environment across the lifespan? Understanding how these elements interact will advance our knowledge of human development, aging, health, and disease. Our functional genomics approach integrates human induced pluripotent stem cell models with CRISPR-based genome engineering to introduce and reverse genetic variation, yielding precision models that can be combined with genetic and pharmacological screens. With this approach, we demonstrated that diverse risk variants share downstream convergent impacts, and that when added together, their combinatorial perturbations yield novel non-additive outcomes that cannot yet be predicted by individual manipulations alone. We seek to understand the genetic regulation of phenotype, and how it is impacted by developmental, cellular, and environmental contexts. Thus, rather than just characterize the impact of trait-associated variants, we seek to uncover modifiers that alter it. For example, we study how genotype-phenotype relationships vary across people and dynamic conditions. Our goal is to decipher the frameworks that buffer genetic risk, in order to confer biological resilience and promote healthy development. We are uniquely positioned to answer critical questions: How does the environment impact genetic regulation? Why are there marked sex effects across many human traits and diseases? What are the molecular mechanisms of resilience, whereby individuals with high genetic risk show no clinical manifestation of disease? Understanding the basic biology governing the complex interplay between genetic variants and the environment will springboard the development of novel, personalized approaches to improve health and prevent disease.
CHAIR: Sarkis MazmanianPhD, Soux Professor, Caltech
PANEL: James Adams,PhD, President’s Professor, Arizona State University; Stephen Walker, Professor, Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine
PANEL: Richard Frye MD, PhD Behavioral Child Neurologist, Neurodevelopmental Precision Medicine, Arthur Krigsman MD, Pediatric Gastroenterologist, Private Practice, New York, Harumi Jyonouchi MD, Allergist- Immunologist, St. Peter’s University Hospital, John Gaitanis MD, Director of Child Neurology, Brown Medical School, Pediatric Neurologist, Epileptologist, Hasbro Children’s Hospital; Ben Marlow MD, Consultant Paediatrician, Colchester General Hospital
Synchrony 2023 Clinical Pearls from Specialists
Director of Child Neurology, Brown Medical School, Pediatric Neurologist, Epileptologist, Hasbro Children’s Hospital
Autism epilepsy and Aggression: The Second Regression Syndrome
A case is presented of a 17 year old boy with responsive nerve stimulation (RNS) who underwent a treatment course of IVIG for severe OCD and Tourette’s syndrome. The RNS leads were placed in the centromedian nucleus of the thalamus bilaterally. The effect of IVIG treatment on RNS detections is reviewed and compared to his treatment response.
Clinical Professor, Lucile Packard Children’s Hospital, Stanford University
Invasive Brain Monitoring in a case of PANDAS treated with IVIG
Behavioral Child Neurologist, Neurodevelopmental Precision Medicine
Follow the Data: Finding The Pearls in Research
HARUMI JYONOUCHI, MD
Allergist- Immunologist, St. Peter’s University Hospital
Autism Spectrum Disorder Inflammatory Subtype and Long COVID






roundtable
discussions
clinical
pearls
researchers’
‘mini’ course
one-on-one
mentoring
research
presentations
industry
updates
Selected panel of Brain Principal Investigator Talks
Postdoctoral Fellow, University of Oklahoma
Infectious Sequelae Associated Anti-Neuronal and Anti-Microbial
Autoantibodies in Autism Spectrum Disorder and PANDAS: Unraveling
Neuroimmune Mechanisms and Comorbidities
Given the overlapping neuropsychiatric symptoms and the presence of anti-neuronal autoantibodies in both ASD and Pediatric Autoimmune Neuropsychiatric Disorder Associated with Streptococcal Infections (PANDAS), our study explored shared mechanisms to identify AE PANDAS subtypes in ASD.
Our investigation into the cross-reactive anti-neuronal and anti-microbial autoantibody responses associated with streptococcal and other childhood infections in ASD and PANDAS revealed that sera from both groups exhibited autoreactive anti-neuronal IgG against dopamine receptors, brain tubulin, and activated calcium/calmodulin-dependent protein kinase II (CaMKII) in human neuronal cells. The autoantibody-mediated dopamine receptor and CaMKII activation was mitigated by absorbing ASD and PANDAS sera with microbial polysaccharides or neuronal antigens. Abnormal antibody responses to streptococcal carbohydrate N-acetyl-beta-D-glucosamine were elevated in
both PANDAS and ASD subsets, alongside observed deficiencies in other microbial polysaccharide antibodies, defining potential immunoglobulin deficiencies and cross-reactivities in these diseases.
Further, we demonstrated shared gene expression signatures and enriched pathways mediated by ASD and PANDAS sera, suggesting similarities and differences in these neurological and psychiatric disorders, and the role of infection. Our findings suggest that molecular mimicry and immune cross-reactivity between host and pathogen contribute to the pathogenesis of infection-mediated autoimmune sequelae, resulting in
the emergence of tic and OCD behaviors in ASD and PANDAS.
Professor, Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine
Developing an evidence-based framework to quantify pathologic status in ASD-associated enterocolitis
JENNIFER FRANKOVICH, MD MS
Clinical Professor, Lucile Packard Children’s Hospital, Stanford University
Talk title: coming soon
Synchrony 2023 Research Talks
Postdoctoral scientist, University of Utah
Calcium transients in microglia drive repetitive form of autistic behaviors
ADRIEN ESHRAGI, MD MSc FACS
Professor of Otolaryngology, Neurosurgery, Pediatrics and Biomedical Engineering, University of Miami
Augmenting mGluR5 activity ameliorates behavior deficits in a transgenic rat model of Autism Spectrum Disorder
Assistant Professor, Feinstein Institutes for Medical Research
Captopril, an Angiotensin-Converting Enzyme (ACE) Inhibitor, attenuates Microglial Activation and Improves Social Behavior in a Mouse Model of Autism Spectrum Disorder
Professor of Psychiatry, University of Missouri-Kansas City
Low Dose Loxapine Resembles an Atypical Antipsychotic with a More Favorable Metabolic Profile Warranting Clinical Trials in ASD
Objectives/Methods: Loxapine 5-15mg daily resembles an atypical antipsychotic on PET studies. Preliminary evidence suggests a superior metabolic profile. We synthesize published evidence.
Results: PET scan studies demonstrated low dose loxapine blocks 5-HT2 and D2 receptors equipotently in vivo; loxapine action resembled atypical antipsychotic actions on dopamine and acetylcholine release in the nucleus accumbens and prefrontal cortex. Loxapine but NOT other antipsychotics produced neural sprouting in human pluripotent stem cells. A prospective open trial of low dose loxapine add-on for irritability in ASD found significant improvement, little or no weight gain, and increases in serum BDNF. A retrospective study of long-term low- dose loxapine in ASD found rates of EPS and TD lower than expected for classical antipsychotics. A retrospective chart review of loxapine 5-10mg daily add-on followed by atypical antipsychotic taper found mean 17-month weight LOSS of 5.7kg, mean BMI REDUCTION of 1.9, and mean triglyceride REDUCTION of 33.7mg/dl (p=.02). One subject discontinued insulin; 2 were tapered off metformin by endocrinologists.
Conclusions: Preliminary evidence justifies immediate pursuit of clinical trials of loxapine in ASD.
Assistant Director, Bloorview Research Institute, Child Neurologist and Senior Clinician Scientist, Holland Bloorview Kids Rehabilitation Hospital, Associate Professor, Department of Pediatrics, University of Toronto
Exploring heterogeneity in neurodevelopment to advance precision health in Autism and related conditions
Assistant Professor, University of Texas
Vagus nerve stimulation as a potential adjuvant to behavioral therapy for autism and other neurodevelopmental disorders
NING QUAN, PHD
Director, Program in Neuroimmunology and Glial Biology, Professor, Stiles-Nicholson Brain Institute, Florida Atlantic University
Neuronal IL-1R1 contributes to Early Life Stress-Induced Long-Lasting Social Interaction Deficits
Our recent publication showed that neuronal IL- 1R1 (nIL-1R1) is important for cognition and social interaction in adult mice. The role of nIL-1R1 in neurodevelopment and related cognitive and affective functions is not known.
Here, we investigated the spatiotemporal expression patterns of nIL-1R1 during neurodevelopment and asked how nIL-1R1 might modulate neurodevelopment. We found that nIL-1R1 expression is very dynamic during brain development and changes dramatically in various brain regions including dentate gyrus of hippocampus (DG), dorsal raphe nucleus (DRN), ventral posteromedial (VPM), and posterolateral thalamic nuclei (VPL). These dynamic changes occurred in brain regions related to neurocircuits involved in the memory, sensory, and mood-regulation functions of the brain in a temporal spatial specific manner.
We assessed ELS- induced social interaction changes and found that nIL-1R1 is necessary for mediating maternal separation (MS, a well-established ELS paradigm) induced deficits in social interaction and social recognition later in life. Molecular analysis revealed a nIL-1R1-dependent acceleration of neuronal maturation in DG, somatosensory cortex, and frontal cortex in the early sensitive period after MS. In addition, we found that -FosB accumulated in granule neurons that express nIL-1R1 in DG after MS whereas this accumulation was not observed in controls. Our behavioral analysis suggests that nIL-1R1 is necessary for MS-induced deficits in social interaction and social recognition later in life.
These findings suggest that: 1) nIL-1R1 is tightly regulated during brain development correlating with its critical role in the control of neuronal maturation by non-neuroinflammatory neuro-immune activities, and 2) nIL-1R1 modulates the ELS-induced stress responses in DG which leads to long-lasting social interaction abnormalities by changing neuronal activity and/or plasticity in the DG
Great conference. I am very excited about the work being done on Microbiome in particular!
Synchrony 2023 Researcher Mini Course
MARIO CAPECCHI, PHD
Nobel Laureate (2007), Biophysicist, Distinguished Professor of Human Genetics, University of Utah
The making of a scientist, an unlikely journey
Luis and Nelly Soux Professor, Caltech
The Gut Microbiome Impacts Symptoms in Shank3 Mice
Assistant Professor of Radiology, Harvard Medical School, Massachusetts General Hospital
Simultaneous PET-MRI reveals choroid plexus alterations in autism spectrum disorder
Director, Stem Cell Program, UCSD, Professor, Departments of Pediatrics and Cellular & Molecular Medicine, UC, San Diego
Title: coming soon
Behavioral Child Neurologist, Neurodevelopmental Precision Medicine
Neuroinflammatory Patterns in Autism Spectrum Disorder Patients with and without Seizures
Individuals with childhood onset seizures without genetic abnormalities tended to have normal Cunningham panels or isolated elevations in anti-tubulin but not Cam Kinase II. Individuals with adolescent onset seizures, childhood onset seizures with genetic abnormalities or subclinical epileptiform discharges tended to have isolated elevations in Cam Kinase II without other autoantibodies elevated.
This study suggests that there are subgroups of children with ASD that have distinct patterns of neuroinflammation markers which fall into subgroups. One of these groups demonstrates elevation in CamKinase II without an identified autoantibody. This suggests that there are yet to be discovered autoantibodies or other inflammatory proteins that are driving Cam Kinase II elevations in this subgroup. Identifying these such proteins can help in the treatment of difficult to control seizures in ASD.
Executive Director , Stiles-Nicholson Brain Institute and Florida Atlantic University College of Medicine
Serotonin autism and the immune system
Child and Adult Psychiatrist, Columbia University
Trials and tribulations: testing new interventions in autism spectrum disorder
Professor and Associate Chair of the Department of Psychiatry and Behavioral Sciences, Stanford University
Developing valid animal models for streamlined translation and clinical impact for autism
Professor – Step Family Endowed Chair; Founding Co-director, Neurodegeneration New Medicines Center, Department of Molecular Medicine, Scripps Research Institute
Talk title coming soon
Synchrony 2023 Invited Talks
Behavioral Child Neurologist, Neurodevelopmental Precision Medicine
Transgenerational Inherited (non-genetic?) metabolic disorders
Recent research is providing evidence of metabolic disorders associated with a majority of children with ASD, including abnormalities in folate, mitochondrial and transmethylation/transsulfuration metabolism. All of these disorders have evidence of transgenerational inheritance without a clear genetic component. These pathways are highly influenced by environmental factors and are prime pathways to study to understand environmental x genetic (probably polygenic) interaction that contribute to ASD.
Studying these abnormalities from the top down (physiological abnormalities) rather than from the bottom up (genetic changes) may help uncover the complicated combination of factors which result in ASD and other neurodevelopmental disorders.
JOHN GAITANIS MD
Director of Child Neurology, Brown Medical School, Pediatric Neurologist, Epileptologist, Hasbro Children’s Hospital
EEG: Past, Present and Future: Implications for Autism
Director, Developmental Clinical Neurophysiology and Neurostimulation Research Program, National Institute of Mental Health, NIH
Transcranial Magnetic Stimulation and Autism
Nobel Laureate (2007), Biophysicist, Distinguished Professor of Human Genetics, University of Utah
Defective Hoxb8 Microglia are Causative for both Chronic Anxiety and OCSD-like Behavior in Mice
ontogenies; Hoxb8 microglia and non-Hoxb8 microglia. Disruption of Hoxb8 or ablation of the Hoxb8 cell lineage gives rise to both chronic anxiety and OCSD-like behavior (trichotillomania, compulsive pathological overgrooming leading to lesions at the sites of overgrooming). There is a strong sex bias for both behavioral pathologies, with females showing much more aggressive disease. By cell transplantation of purified, cell sorted Hoxb8 microglia progenitors derived from Wt or Hoxb8 mutant mice we were able to demonstrate causation of defective Hoxb8 microglia for both behavioral pathologies. More recently we have demonstrated that optogenetic activation of Hoxb8 microglia in specific regions of the brain induces higher levels of anxiety, grooming or both. Further, these experiments have demonstrated that Hoxb8 microglia function to reduce anxiety and grooming (function as brakes) whereas non-Hoxb8 microglia function to increase these behaviors (function as an accelerator). Now we know why mammals have two populations of microglia.
Distinguished professor of neurosciences and pediatrics, UC San Diego
A Clinical Trial of Cannabidiol for Autism: What We’ve found and What I’ve Learned
STEPHEN WALKER, PHD
Professor, Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine
ARTHUR KRIGSMAN, MD
Pediatric Gastroenterologist, Private Practice, New York
Significant improvements (gastrointestinal, behavioral, cognitive) following standard-of-care treatment in children with ASD-associated enterocolitis: A longitudinal assessment
Associate Professor, University of Pennsylvania
From Chasing My Cure to Every Cure
Synchrony 2023 Industry Updates
JOHN SLATTERY
Chief Executive Officer, BIORosa technologies
An update from BioROSA’s Metabolic Autism Prediction (MAP) study
Research conducted over the past few decades have consistently shown dysfunctional metabolic signals involving redox metabolism in patients diagnosed with Autism Spectrum Disorder (ASD). To date there have been no double-blind case/control studies that seek to determine if abnormalities in 1-carbon folate metabolism, cellular methylation, and redox abnormalities are predictive of all forms of ASD compared to other types of developmental concerns (DD) that present early in life. BioROSA Technologies recently completed the first multicenter prospective double-blind case/control trial in which 163 children aged 18-60 months were enrolled. All children had a fasting blood draw and underwent diagnostic evaluation for ASD using DSM-5 criteria for diagnosis. All children had an assessment that included the Autism Diagnostic Observation Schedule (ADOS), Mullen Scales of Early Learning (MSEL), Vineland Adaptive Behavior Scales (VABS), and a full medical history and physical exam. The primary endpoint for the trial was to determine if metabolic biomarkers using a machine learning algorithm could accurately differentiate ASD from DD with accuracy >/= 80%. BioROSA test performance has demonstrated a positive signal in the MAP pilot trial. Details will be discussed.
Chief Executive Officer, Cell El
Can Serum Protein Biomarkers Identify Children at High Risk
for Autism Spectrum Disorder?
Senior Vice President of Pharmaceutical Development, Eumentis Therapeutics
& RANDALL MARSHALL
Chief Medical Officer, Eumentis Therapeutics
EM-113 for autism spectrum disorder patients with elevated glutamate in the anterior cingulate cortex by MR Spectroscopy
controlled trial from MGH showing memantine is highly effective for improving social deficits in this subgroup of children (Gagan Joshi MD, MGH). Prior large, controlled trials’ failure with memantine were likely due to under-dosing, heterogeneity of ASD patients enrolled, and lack of a precision biomarker.Results from the MGH trial and the EuMentis planned trial will be presented and discussed.
President, Gut-Brain Axis Therapeutics
& KHEMLAL NIRMALKAR, PHD
Research Scientist, Biodesign center for Health Through Microbiomes, Arizona State University
Microbiota Transplant Therapy for Adults with Autism
Chief Technology Officer, JelikaLite
Transcranial Photobiomodulation for treatment of core symptoms of ASD in young children
Medical Director of Pediatric Developmental Disabilities, Kaiser Permanente NCAL
MAUDE